Conducting Periodic HPLC Performance Checks: Keys to Reliable System Operation

Conducted by John W. Dolan, PhD - LC Resources, Inc.

June 14, 2007

HPLC systems can produce very reliable results, or they can seem to produce nothing but problems. Users that have the best results perform periodic performance checks to make sure the system is operating properly and to help anticipate problems that may occur in the future.

Some of these tests are mandated or strongly suggested by regulatory agencies, as in the familiar Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) tests. John Dolan will share some key laboratory practices that he has gleaned from more than 35 years in chromatography. This seminar will present a handful of tests that are easy to run, yet help provide security that the HPLC system is working properly. Run on a semi-annual basis, these can greatly enhance the reliability of your system and thus the data it produces.

This seminar will be conducted on the telephone and is presented by John Dolan PhD, a leading expert in HPLC. In this unique audio seminar format, lasting just one hour, you will be able to follow along with a PDF presentation, ask questions, and interact with Dr. Dolan.

A detailed outline of this seminar and Dr. Dolan's bio follow below.

Conducting Periodic HPLC Performance Checks: Keys to Reliable System Operation

Part I - Introduction

  • What is IQ, OQ, and PQ
  • System suitability testing

 

Part III - Chromatographic checks

  • Detector wavelength accuracy
  • Autosampler precision, carryover, and linearity
  • All-up system test
  • Documentation

Part II - Pump and Detector Checks

  • Power up diagnostics
  • Pump tests: siphon, pressure bleed- down, and flow rate
  • Detector tests: noise and drift

Part V - Questions and Answers

  • Dr. Dolan field your questions live on the phone. You will hear all questions and answers from all participants of this seminar.

Part III - On-line mixing checks

  • Gradient step-test & GPV test
  • Gradient linearity & dwell volume
  • Examples of test failures

     

 

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Get the CD and handout
LC070614E - CD and Handout materials
Conducting Periodic HPLC Performance Checks
CD price includes HANDOUT MATERIALS and audio CD. CD will be shipped three weeks after the seminar
$397.00 Add to Cart

Instructor

John W. Dolan, Ph.D.
Dr. John Dolan is most widely known as the author of LC/GC’s popular LC Troubleshooting column. As editor of this column for over 20 years, Dr. Dolan has contributed more than 250 installments of practical advice to practicing chromatographers. John has written over 100 technical papers, many of which support the development and practical application of gradient elution LC. His book (with Lloyd Snyder),Troubleshooting LC Systems, is a standard reference in laboratories worldwide. Just off the press is a second book with Lloyd Snyder, High-Performance Gradient Elution. He is the 2002 recipient of the prestigious Palmer Award given by the Minnesota Chromatography Forum and the 2007 Dal Nogare Award presented at the Pittsburgh Conference by the Delaware Chromatography Forum.

Dr. Dolan managed a regional contract research laboratory for 11 years for LC Resources and later Bioanalytical Systems, Inc. The lab, which he helped found in 1988, specializes in the development, validation, and application of LC-MS/MS methods to determine pharmaceutical compounds in biological matrices. In addition, he is one of the founders of LC Resources, Inc., a company dedicated to training chromatographers around the world. As an instructor for LCR and the American Chemical Society, John has instructed more than 10,000 students in practical LC techniques.

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