Method Development Strategies for Reversed-Phase HPLC: How to Get the Most Out of Your Method Development Efforts

Conducted by John W. Dolan and Tom Jupille - LC Resources, Inc.

March 2, 2007

Method development for liquid chromatography often is surrounded by an aura of mystery, suggesting that only the insiders know the secrets of how to develop good methods. Too many times, method development is simply a series of trial-and-error experiments in search for a workable method. Unfortunately, this approach often results in many trials, many errors, and lots of hope, but less-than-satisfactory methods.

The instructors for this audio seminar are John Dolan and Tom Jupille. Between them, they have more than 60 years of HPLC experience, including more than 40 years of teaching HPLC techniques to more than 15,000 students worldwide.

Dr. Dolan has designed LC equipment, developed methods, managed a contract research laboratory, and written over 100 papers on LC. Many know him well through his LC Troubleshooting column appearing monthly in LCGC magazine for more than 20 years.

Tom Jupille managed HPLC applications laboratories for well-known LC supplies and instrument companies, training users on the best techniques to take advantage of their instruments. Tom is recognized as the moderator of the popular Chromatography Forum on-line discussion group, providing a resource for solving practical problems many chromatographers encounter.

A detailed outline and Dolan's and Jupille's bios follow below.

Method Development Strategies for Reversed-Phase HPLC
How to Get the Most Out of Your Method Development Efforts

Part I - Setting appropriate goals

  • What is reasonable and what isn't?
  • How much do I have to know in advance?

Part IV - Knowing when to stop

  • What to look for in a good method
  • When is it time to give up?

Part II - Determining which parameters to change

  • Which variable is most likely to get me results?
  • Are there changes that I should avoid?

Part V - Questions and Answers

  • John or Tom will field your questions live on the phone. You will hear all questions and answers from all participants of this teleseminar.

Part III - Using a structured approach

  • How do I get started?
  • How do I choose the next experiment?
  

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LC070302E - CD and Handout materials
Method Development Strategies for Reversed-Phase HPLC
CD price includes HANDOUT MATERIALS and audio CD. CD will be shipped three weeks after the seminar		 $397.00

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Instructors

John W. Dolan, Ph.D.
Dr. John Dolan is most widely known as the author of LC/GC’s popular LC Troubleshooting column. As editor of this column for over 20 years, Dr. Dolan has contributed more than 250 installments of practical advice to practicing chromatographers. John has written over 100 technical papers, many of which support the development and practical application of gradient elution LC. His book (with Lloyd Snyder),Troubleshooting LC Systems, is a standard reference in laboratories worldwide. Just off the press is a second book with Lloyd Snyder, High-Performance Gradient Elution. He is the 2002 recipient of the prestigious Palmer Award given by the Minnesota Chromatography Forum and the 2007 Dal Nogare Award presented at the Pittsburgh Conference by the Delaware Chromatography Forum.

Dr. Dolan managed a regional contract research laboratory for 11 years for LC Resources and later Bioanalytical Systems, Inc. The lab, which he helped found in 1988, specializes in the development, validation, and application of LC-MS/MS methods to determine pharmaceutical compounds in biological matrices. In addition, he is one of the founders of LC Resources, Inc., a company dedicated to training chromatographers around the world. As an instructor for LCR and the American Chemical Society, John has instructed more than 10,000 students in practical LC techniques.

Thomas H. Jupille, M.S.
Tom Jupille has been a practicing chromatographer for more than 30 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in the late ‘70s. His career has focused on instrument and column development, and user support, providing a broad foundation of practical experience to call on as an instructor.

Over the past 20 years, Tom Jupille has presented courses and seminars in the field of chromatography to more than 5000 students. In addition to teaching, he has been involved in the development and support of computer modeling techniques for chromatography method development. He is President of LC Resources, a Consulting Editor for LCGC, and associate director of the California Separation Science Society (CASSS). Tom is the founder and moderator of Chromatography Forum (www.chromforum.com), an on-line discussion group focused on providing practical support to practicing chromatographers.

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