HPLC Diagnostics: What to Do When Things Go Wrong!
Conducted by Tom Jupille - LC Resources, Inc.
November 30, 2007
As HPLC systems have become more and more "appliance-like", we become increasingly used to having on-board computer control and diagnostics. The fact is, however, that a liquid chromatograph is not a microwave oven; problems requiring operator intervention can and do occur. On-board diagnostics are great, as far as they go. But they can't tell you whether your problem is due to hardware malfunction or separation chemistry problems. This one-hour seminar will give you the tools you need to tell the difference! We'll look at a range of simple diagnostic tests that can help you answer questions like these:
- what's causing my baseline to drift?
- where are these "garbage" peaks coming from?
- is my column dying because of garbage in the sample or a corrosive mobile phase?
- are my repeatability problems coming from sample prep or separation conditions?
- how come I'm seeing negative peaks (and how do I get rid of them)?
In addition, this seminar will discuss a number of common-sense diagnostic tips and tricks that you can use when you REALLY have to track down the source of the problems such as:
- column contamination
- packing deterioration
- late-eluters
- gradient proportioning problems
- gradient garbage peaks
- flow rate problems
The instructor for this seminar is Tom Jupille. Tom managed HPLC applications laboratories for well-known LC supplies and instrument companies, training users on the best techniques to take advantage of their instruments. Tom is recognized as the moderator of the popular Chromatography Forum on-line discussion group, providing a resource for solving practical problems many chromatographers encounter.
In this unique web seminar format, lasting just one hour, you will be able to follow along with a PDF file, ask questions, and interact with Tom. Mr. Jupille's qualifications can be found below.
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All registrations include handout materials. Once you’re registered, you will receive via e-mail (the e-mail address you provide in your registration) the web location to download the handout materials.
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| Get the CD and handout LC071130E - CD and Handout materials HPLC Diagnostics CD price includes HANDOUT MATERIALS and audio CD. CD will be shipped three weeks after the seminar |
$397.00 |  |
Instructor
Thomas H. Jupille, M.S.
Tom Jupille has been a practicing chromatographer for more than 30 years, during which he has written more than 30 papers on chromatography and related subjects. He worked primarily in gas chromatography in the late ‘60s, switching to thin-layer chromatography in the early ‘70s and then to HPLC and ion chromatography in the late ‘70s. His career has focused on instrument and column development, and user support, providing a broad foundation of practical experience to call on as an instructor.
Over the past 20 years, Tom Jupille has presented courses and seminars in the field of chromatography to more than 5000 students. In addition to teaching, he has been involved in the development and support of computer modeling techniques for chromatography method development. He is President of LC Resources, a Consulting Editor for LCGC, and associate director of the California Separation Science Society (CASSS). Tom is the founder and moderator of Chromatography Forum (www.chromforum.com), an on-line discussion group focused on providing practical support to practicing chromatographers.
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